Abstract: Three bacteria were screened out based on the actual conditions in Qigequan oil field of Qinghai province. The salt and heat resistance of them obtained in lab indicated that they could be cultlvated in a great amount at 6OCand 120000ppm. After crude oil was treated by means of them, the viscosity, paraffin content and freezing point were all de-creased. Indoor simulation test indicated that more than 20% oil could be recovered by means of microbe oil recovery method after water flooding’ The field testing of huff and puff injection of microbe in individual well indicated that this technology could effectively remove paraffin in and near well bore, decrease oil viscosity, improve oil mobillty, increase forma-tion energy and increase oil output. Microbe oil recovery technology is simple in construc-tion, low in cost, does not contaminate formation and environment, and is a kind of econom-ic and effective EOR method.