Abstract: With the rapid development of native microbial enhanced oil recovery technique in China recent decade, paraffin removal and prevention by microbial and huff puff with single well have became mature explore technologies in oil fields and have been wide scale used in many oilfields taking as production maintain and stimulation measures, respectively. However, the existence of mechanism misunderstanding and technique optimizing still need further study to enhance the effect of treatment and efficiency. Meanwhile, the microbial flooding technology is still on pilot stage, although more than 20 field trials have been carried out. There is no consensus of opinion in microbial flooding and the orientation of technological development is still obscure so that there are difficulties on technology extension. Studies, field tests and applications in recent decade about microbial technology were discussed in this paper, and the several key points in microbial flooding were discussed in detail. On the basis of analyzing on-site experiment patterns and developing trend, some problems that need to pay attention in next research and development in the future have been proposed.